Advances In Instrumentation Research Articles

THE CONTRIBUTION OF THE DE FILIPPI EXPEDITION (1913–1914) TO THE GEOLOGICAL KNOWLEDGE OF THE AKSAI CHIN REGION (WESTERN HIMALAYA)

ABSTRACT Between the years 1913 to 1914 the De Filippi Expedition, led by the Italian explorer and mountaineer Filippo De Filippi (1869–1938), traversed the Himalayan Range from Kashmir to Baltistan and Xinjiang. Exploring mostly unknown territories, traveling more than 2000 kilometers, and equipped with advanced instruments for geodetic and meteorological measurements, the expedition members surveyed, mapped and collected rocks and fossils including from the remote Aksai Chin region. After almost two years, the expedition returned to Italy, however, publication of the findings was delayed due to the outbreak of the First World War. The fossil material and syntheses of the geology and stratigraphy of the area represented unique documents on this sector of Western Tibet, with the Cretaceous Period being documented by the expedition for the first time in the whole Trans-Himalayan Range. The Aksai Chin region remains poorly explored even today, and the De Filippi collection which is housed in the Natural History Museum of the University of Florence now represents one of the few records available for reconstructing the stratigraphy of the area. It constitutes a valuable resource regarding the history of the scientific exploration and understanding of the geology and paleontology of the region, providing a unique window into a very remote and fascinating part of the world.

Development and evaluation of RFID-integrated endoscopic clips for laparoscopic surgery marking.

As advancements in surgical instruments and techniques continue to evolve, minimally invasive surgery has become increasingly preferred as a means of reducing patient pain and recovery time. However, one major challenge in performing minimally invasive surgery for early gastrointestinal cancer is accurately identifying the location of the lesion. This is particularly difficult when the lesion is confined to the lumen of the intestine and cannot be visually confirmed from the outside during surgery. In such cases, surgeons must rely on CT or endoscopic imaging to locate the lesion. However, if the lesion is difficult to identify with these images or if the surgeon has less experience, it can be challenging to determine its precise location. This can result in an excessive resection margin, deviating from the goal of minimally invasive surgery. To address this challenge, researchers have been studying the development of a marker for identifying the lesion using a radio-frequency identification (RFID) system. One proposed method for clinical application of this detection system is to attach an RFID tag to an endoscopic hemostatic clip and fix it to the intended position, providing a stable marker for the inner wall of the organ. This approach has the potential to improve the accuracy and effectiveness of minimally invasive surgery for early gastrointestinal cancer. In the development of a marker for identifying gastrointestinal lesions using a radio-frequency identification (RFID) system, the shape of the clip and suitable materials for attaching the RFID tag were determined through finite element method (FEM) analysis. A prototype of the clip was then fabricated and ex-vivo experiments were conducted using porcine intestine to evaluate the stability of the clip in relation to its position. To further evaluate the performance of the RFID-integrated clip in vivo, the clip was placed in the gastric wall of the stomach of anesthetized porcine using an endoscopic instrument. The clip was then detected using a RFID detector designed for laparoscopic approach. And later, the accuracy of detection was confirmed by incising the lesion. The design and fabrication of a clip with varying thicknesses using STS316 and STS304 stainless steel were accomplished using the results of finite element method analysis. The stability of the clip was evaluated through ex-vivo experiments, showing it to be a viable option. In-vivo experiments were performed on anesthetized porcine, in which the RFID-integrated clip was placed in the gastric wall and detected using a custom-made RFID detector. The resection margin, measured at about 30 mm from the detector position, was accomplished with low error. These findings indicate the feasibility and efficacy of using an RFID-integrated clip as a marker in minimally invasive surgery for the identification of gastrointestinal lesions. The study evaluated the feasibility of using stainless steel clips for lesion detection in endoscopic surgery using computer-aided engineering analysis and ex-vivo experimentation. Results showed that STS304 was suitable for use while STS316L was not. The ex-vivo experiments revealed that the clip holding force and tissue retention length varied depending on the location of attachment. In-vivo experiments confirmed the accuracy and usefulness of the RFID lesion detection system. However, challenges remain for its use in clinical field, such as ensuring the stability of the clip and the safe attachment of the RFID tag, which requires further research for commercialization.

An Update on MR Spectroscopy in Cancer Management: Advances in Instrumentation, Acquisition, and Analysis.

MR spectroscopy (MRS) is a noninvasive imaging method enabling chemical and molecular profiling of tissues in a localized, multiplexed, and nonionizing manner. As metabolic reprogramming is a hallmark of cancer, MRS provides valuable metabolic and molecular information for cancer diagnosis, prognosis, treatment monitoring, and patient management. This review provides an update on the use of MRS for clinical cancer management. The first section includes an overview of the principles of MRS, current methods, and conventional metabolites of interest. The remainder of the review is focused on three key areas: advances in instrumentation, specifically ultrahigh-field-strength MRI scanners and hybrid systems; emerging methods for acquisition, including deuterium imaging, hyperpolarized carbon 13 MRI and MRS, chemical exchange saturation transfer, diffusion-weighted MRS, MR fingerprinting, and fast acquisition; and analysis aided by artificial intelligence. The review concludes with future recommendations to facilitate routine use of MRS in cancer management. Keywords: MR Spectroscopy, Spectroscopic Imaging, Molecular Imaging in Oncology, Metabolic Reprogramming, Clinical Cancer Management © RSNA, 2024.

Multidisciplinary surgical considerations for en bloc resection of sacral chordoma: review of recent advances and a contemporary single-center series.

Contemporary management of sacral chordomas requires maximizing the potential for recurrence-free and overall survival while minimizing treatment morbidity. En bloc resection can be performed at various levels of the sacrum, with tumor location and volume ultimately dictating the necessary extent of resection and subsequent tissue reconstruction. Because tumor resection involving the upper sacrum may be quite destabilizing, other pertinent considerations relate to instrumentation and subsequent tissue reconstruction. The primary aim of this study was to survey the surgical approaches used for managing primary sacral chordoma according to location of lumbosacral spine involvement, including a narrative review of the literature and examination of the authors' institutional case series. The authors performed a narrative review of pertinent literature regarding reconstruction and complication avoidance techniques following en bloc resection of primary sacral tumors, supplemented by a contemporary series of 11 cases from their cohort. Relevant surgical anatomy, advances in instrumentation and reconstruction techniques, intraoperative imaging and navigation, soft-tissue reconstruction, and wound complication avoidance are also discussed. The review of the literature identified several surgical approaches used for management of primary sacral chordoma localized to low sacral levels (mid-S2 and below), high sacral levels (involving upper S2 and above), and high sacral levels with lumbar involvement. In the contemporary case series, the majority of cases (8/11) presented as low sacral tumors that did not require instrumentation. A minority required more extensive instrumentation and reconstruction, with 2 tumors involving upper S2 and/or S1 levels and 1 tumor extending into the lower lumbar spine. En bloc resection was successfully achieved in 10 of 11 cases, with a colostomy required in 2 cases due to rectal involvement. All 11 cases underwent musculocutaneous flap wound closure by plastic surgery, with none experiencing wound complications requiring revision. The modern management of sacral chordoma involves a multidisciplinary team of surgeons and intraoperative technologies to minimize surgical morbidity while optimizing oncological outcomes through en bloc resection. Most cases present with lower sacral tumors not requiring instrumentation, but stabilizing instrumentation and lumbosacral reconstruction are often required in upper sacral and lumbosacral cases. Among efforts to minimize wound-related complications, musculocutaneous flap closure stands out as an evidence-based measure that may mitigate risk.

Productivity of operating room from viewpoint operating room nurses in Iran: A national cross-sectional study

BackgroundProductivity refers to the maximum use of human resources in service provision. Due to the existence of advanced equipment and instruments for surgery, the operating room is one of the most complex and income-generating sections of a hospital. Since the improvement of operating room nurses’ productivity can lead to enhanced hospital productivity, this study was done to determine the productivity rate of operating room nurses in Iran. MethodsThis cross-sectional study was done on 533 operating room nurses from 8 metropolises in Iran in 2022. The sampling method in this research was multistage. The data collection instruments were Hersy & Belanchard & Gold.Smith productivity questionnaire and demographic characteristics. After receiving the ethics code, data collection was done for three months. Descriptive and inferential analyses, including independent t-tests and analysis of variance, were used for data analysis. The collected data were analyzed by SPSS 18. P-value <0.05 was considered statistically significant. ResultsThe mean age of the participants was 29.83±5.94 and 421 of the participants were women. The mean and SD for total productivity in operating room nurses was obtained at 60.83 ± 12.78, below average. Data analysis shows that total productivity was significantly associated with marital status (P-value=0.035) and working experience (P-value=0.019). ConclusionConsidering the low mean productivity rate of operating room nurses, researchers recommend that hospital managers pay more attention to the productivity of operating room nurses by knowing the influential factors and implementing proper and high-quality management.

Passive high explosive neutron inspection (PHENIX): a new method to confirm the presence or absence of high explosives for nuclear treaty verification

Advanced instruments and methods need to be developed now to create a technical basis to support the negotiation of future nuclear arms control treaties. One new capability that is anticipated is the ability to confirm either the declared presence or declared absence of high explosive (HE) material in the presence of special nuclear material (SNM). Towards this goal, Passive HE Neutron Inspection (PHENIX) has been developed and demonstrated as a method for confirming the presence or absence of HE in the presence of plutonium. The method exploits the inherent presence of neutrons associated with the decay of plutonium as an internal probe source for performing prompt gamma-ray neutron activation analysis (PGNAA), searching for the presence of HE as revealed by the emission of characteristic gamma rays following neutron absorption in hydrogen and nitrogen which are building blocks of present-day, military-grade HE. Tests using stoichiometrically-correct hemishells of mock HE with plutonium show that a system can be expected to positively confirm the presence or absence of these signatures, supporting determination of HE presence or absence with Pu, in a few hours. To protect other potentially sensitive gamma-ray signatures from a treaty accountable item, an analog information barrier has been conceptualized and tested which physically prevents the collection of gamma-ray spectral data outside of user selected energy windows strategically chosen to view only narrow spectral regions corresponding to the hydrogen (2223.2 keV) and nitrogen (9807.2 keV, 10,318.2 keV, and 10,829.2 keV) PGNAA signatures.

MOHS SURGERY

Mohs surgery is a highly specialized procedure used to treat skin cancer, especially basal cell carcinoma and squamous cell carcinoma. This technique is known for its high cure rate and preservation of healthy tissue around the affected area. This study aims to review and address the most recent updates in the Mohs surgery technique, including advances in equipment and instruments, improved treatment protocols and post-surgical reconstruction techniques, while also discussing the growing importance of this procedure in contemporary dermatological practice and its positive impact on patients’ quality of life. Mohs surgery involves the surgical removal of thin layers of cancerous tissue, followed by immediate microscopic analysis of the margins of the removed tissue. This allows precise identification of the tumor margins, ensuring complete removal of the cancer while preserving as much healthy tissue as possible. Recent updates to the technique include the use of advanced imaging techniques, such as dermoscopy and confocal microscopy, to aid in the precise identification of tumor margins prior to surgery. In addition, the development of more effective surgical reconstruction methods has improved aesthetic and functional results for patients. It is therefore concluded that Mohs surgery remains an effective option for the treatment of skin cancer, offering high cure rates and preservation of healthy tissue. Updates to the technique, including improvements in diagnostic accuracy and reconstruction options, have contributed to increasingly better results for patients undergoing this procedure.

Диагностика сигналов ЭКГ на основе цифровой обработки и оптимизированной модели трансформера

Heartbeat disorders are considered one of the main maladies that cause mortality. Therefore, their precocious diagnosis via ECG signal is critical for introducing prompt therapy. The advanced automatic classification of ECG signals has the potential to save cardiologists a tremendous amount of time while simultaneously decreasing the chance of misdiagnosis. The dilemma of massive parameters is troubling the current methods of ECG signal classification. Most recent methods exhibit inadequate performance for diagnosing ECG signals in the inter-patient mode. In an attempt to deal with the above limitations, this study offers an innovative, efficient, and end-to-end model. The suggested model uses the optimized transformer framework to classify the heartbeats according to the "Association for the Advancement of Medical Instrumentation, AAMI," and obeys the inter-patient setting. We constructed an efficient architecture called the optimized network to substitute the Self Attention Unit (SAU) in the encoder part of the transformer model. The suggested model, which includes an optimized network, outperforms the SAU-based transformer model and requires fewer computations. A robust embedding architecture based on a Convolutional Neural Network (CNN) with a Squeeze and Excitation (SE) network-based attention scheme that has been used for weighting the Local Heartbeat Shape Pattern (LHSP) features is presented. The introduced model exceeds the state-of-the-art. An extensive test has been done to compare the achievements of the suggested model with those of the cardiologists. The results proved the closeness of their performances.

Electronic Visualization Laboratory's 50th Anniversary Retrospective: Look to the Future, Build on the Past

Abstract September 2023 marks the 50th anniversary of the Electronic Visualization Laboratory (EVL) at University of Illinois Chicago (UIC). EVL's introduction of the CAVE Automatic Virtual Environment in 1992, the first widely replicated, projection-based, walk-in, virtual-reality (VR) system in the world, put EVL at the forefront of collaborative, immersive data exploration and analytics. However, the journey did not begin then. Since its founding in 1973, EVL has been developing tools and techniques for real-time, interactive visualizations—pillars of VR. But EVL's culture is also relevant to its successes, as it has always been an interdisciplinary lab that fosters teamwork, where each person's expertise contributes to the development of the necessary tools, hardware, system software, applications, and human interface models to solve problems. Over the years, as multidisciplinary collaborations evolved and advanced scientific instruments and data resources were distributed globally, the need to access and share data and visualizations while working with colleagues, local and remote, synchronous and asynchronous, also became important fields of study. This paper is a retrospective of EVL's past 50 years that surveys the many networked, immersive, collaborative visualization and VR systems and applications it developed and deployed, as well as lessons learned and future plans.

Dynamics of manual impaction instruments during total hip arthroplasty.

Manual impaction, with a mallet and introducer, remains the standard method of installing cementless acetabular cups during total hip arthroplasty (THA). This study aims to quantify the accuracy and precision of manual impaction strikes during the seating of an acetabular component. This understanding aims to help improve impaction surgical techniques and inform the development of future technologies. Posterior approach THAs were carried out on three cadavers by an expert orthopaedic surgeon. An instrumented mallet and introducer were used to insert cementless acetabular cups. The motion of the mallet, relative to the introducer, was analyzed for a total of 110 strikes split into low-, medium-, and high-effort strikes. Three parameters were extracted from these data: strike vector, strike offset, and mallet face alignment. The force vector of the mallet strike, relative to the introducer axis, was misaligned by an average of 18.1°, resulting in an average wasted strike energy of 6.1%. Furthermore, the mean strike offset was 19.8 mm from the centre of the introducer axis and the mallet face, relative to the introducer strike face, was misaligned by a mean angle of 15.2° from the introducer strike face. The direction of the impact vector in manual impaction lacks both accuracy and precision. There is an opportunity to improve this through more advanced impaction instruments or surgical training.

Portable Instrumentation for Ambient Ionization and Miniature Mass Spectrometers.

We critically evaluate the current status of portable mass spectrometry (pMS), particularly where this aligns with ambient ionization. Assessing the field of pMS can be quite subjective, especially in relation to the portable aspects of design, deployment, and operation. In this review, we discuss what it means to be portable and introduce a set of criteria by which pMS and ambient ionization sources can be assessed. Moreover, we consider the recent literature in terms of the most popular and significant advances in portable instrumentation for ambient ionization and miniature mass spectrometers. Finally, emerging trends and exciting future prospects are discussed and some recommendations are offered.

Small-scale magnetic flux emergence preceding a chain of energetic solar atmospheric events

Advancements in instrumentation have revealed a multitude of small-scale extreme-ultraviolet (EUV) events in the solar atmosphere and considerable effort is currently undergoing to unravel them. Our aim is to employ high-resolution and high-sensitivity magnetograms to gain a detailed understanding of the magnetic origin of such phenomena. We used coordinated observations from the Swedish 1-m Solar Telescope (SST), the Interface Region Imaging Spectrograph ( and the Solar Dynamics Observatory ( to analyze an ephemeral magnetic flux emergence episode and the following chain of small-scale energetic events. These unique observations clearly link these phenomena together. The high-resolution (0 $) magnetograms obtained with SST/CRISP allowed us to reliably measure the magnetic field at the photosphere and to detect the emerging bipole that caused the subsequent eruptive atmospheric events. Notably, this small-scale emergence episode remains indiscernible in the lower resolution SDO/HMI magnetograms (0 $). We report the appearance of a dark bubble in related to the emerging bipole, a sign of the canonical expanding magnetic dome predicted in flux emergence simulations. Evidence of reconnection are also found, first through an Ellerman bomb and later by the launch of a surge next to a UV burst. The UV burst exhibits a weak EUV counterpart in the coronal SDO/AIA channels. By calculating the differential emission measure (DEM), its plasma is shown to reach a temperature beyond 1 MK and to have densities between the upper chromosphere and transition region. Our study showcases the importance of high-resolution magnetograms in revealing the mechanisms that trigger phenomena such as EBs, UV bursts, and surges. This could hold implications for small-scale events similar to those recently reported in the EUV using Solar Orbiter. The finding of temperatures beyond 1 MK in the UV burst plasma strongly suggests that we are examining analogous features. Therefore, we recommend caution when drawing conclusions from full-disk magnetograms that lack the necessary resolution to reveal their true magnetic origin.

Fibers and Forensics : A Review of Textile Analysis in Stab Wound Cases

This paper covers the complex field of forensic textile stabbing analysis, including its past growth, methods, case studies, applications, and database use. The evaluation begins with an outline of its historical background and proceeds to examine diverse analytical techniques and equipment essential for proficient analysis. It follows the development of stab analysis from simple visual inspections to advanced instruments. The review clarifies the critical function of stab analysis in criminal investigations via the lens of case studies and academic publications. The relationship between stab analysis and other forensic specialties is also examined, with a focus on cooperation with material engineering domains. The study emphasizes the value of stab analysis in modern forensic science by demonstrating its potential. This article offers insights to improve analysis procedures and further the cause of justice, making it a useful tool for forensic professionals, advocates, and researchers alike. Through a combination of historical viewpoints, methodological developments, and multidisciplinary efforts, the review facilitates innovation in conventional forensic technology, which in turn helps to resolve complex criminal cases.

Probing dark matter with adaptive-optics based flux ratio anomalies: photometric and astrometric precision

Abstract Strong gravitational lensing is a powerful probe of the distribution of matter on sub-kpc scales. It can be used to test the existence of completely dark subhalos surrounding galaxies, as predicted by the standard cold dark matter model, or to test alternative dark matter models. The constraining power of the method depends strongly on photometric and astrometric precision and accuracy. We simulate and quantify the capabilities of upcoming adaptive optics systems and advanced instruments on ground-based telescopes, focusing as an illustration on the Keck Telescope (OSIRIS + KAPA, LIGER + KAPA) and the Thirty Meter Telescope (TMT; IRIS + NFIRAOS). We show that these new systems will achieve dramatic improvements over current ones in both photometric and astrometric precision. Narrow line flux ratio errors below 2 per cent, and submilliarcsecond astrometric precision will be attainable for typical quadruply imaged quasars. With TMT, the exposure times required will be of order a few minutes per system, enabling the follow-up of 100-1000 systems expected to be discovered by the Rubin, Euclid, and Roman Telescopes.

Root Canal Instrumentation: Current Trends and Future Perspectives.

The evolution of root canal instrumentation techniques has significantly impacted the field of endodontics, enhancing both the efficiency and outcomes of treatments. This review outlines the transition from manual to mechanical and rotary instruments, highlighting the role of nickel-titanium (NiTi) alloys and smart technologies in advancing procedural precision and reducing patient discomfort. Key historical developments and technological innovations, such as digital imaging and navigation systems, are explored for their contributions to improved clinical outcomes and patient satisfaction. Additionally, the review addresses the challenges presented by the complex anatomy of the root canal system and the advent of current instrumentation techniques. The potential of emerging trends, including artificial intelligence and advances in materials science, is discussed in the context of future endodontic practices. Despite the progress, challenges related to using advanced instrumentation methods, ethical considerations, and the cost factor of new technologies persist. The present review underscores the ongoing need for research and development to further refine root canal instrumentation techniques, ensuring that advancements in endodontic care remain patient-centered and accessible.

HYPERNETS: a network of automated hyperspectral radiometers to validate water and land surface reflectance (380–1680 nm) from all satellite missions

Satellites are now routinely used for measuring water and land surface reflectance and hence environmentally relevant parameters such as aquatic chlorophyll a concentration and terrestrial vegetation indices. For each satellite mission, radiometric validation is needed at bottom of atmosphere for all spectral bands and covering all typical conditions where the satellite data will be used. Existing networks such as AERONET-OC for water and RadCalNet for land provide vital information for validation, but (AERONET-OC) do not cover all spectral bands or (RadCalNet) do not cover all surface types and viewing angles. In this Perspective Article we discuss recent advances in instrumentation, measurement methods and uncertainty estimation in the field of optical radiometry and put forward the viewpoint that a new network of automated hyperspectral radiometers is needed for multi-mission radiometric validation of water and land surface reflectance. The HYPERNETS federated network concept is described, providing a context for research papers on specific aspects of the network. This network is unique in its common approach to both land and water surfaces. The common aspects and the differences between land and water measurements are explained. Based on early enthusiasm for HYPERNETS data from validation-oriented workshops, it is our viewpoint that this new network of automated hyperspectral radiometers will be useful for multi-mission radiometric validation of water and multi-angle land surface reflectance. The HYPERNETS network has strong synergy with other measurement networks (AERONET, AERONET-OC, RadCalNet, FLUXNET, ICOS, skycam, etc.) and with optional supplementary measurements, e.g., water turbidity and fluorescence, land surface temperature and soil moisture, etc.

Procedures from samples to sulfur isotopic data: A review.

Sulfur isotopes have been widely used to solve some key scientific questions, especially in the last two decades with advanced instruments and analytical schemes. Different sulfur speciation and multiple isotopes analyzed in laboratories worldwide and in situ microanalysis have also been reported in many articles. However, methods of sampling to measurements are multifarious, and occasionally some inaccuracies are present in published papers. Vague methods may mislead newcomers to the field, puzzle readers, or lead to incorrect data-based correlations. We have reviewed multiple methods on sulfur isotopic analyses from the perspectives of sampling, laboratory work, and instrumental analysis in order to help reduce operational inhomogeneity and ensure the fidelity of sulfur isotopic data. We do not deem our proposed solutions as the ultimate standard methods but as a lead-in to the overall introduction and summary of the current methods used. It has been shown that external contamination and transformation of different sulfur species should be avoided during the sampling, pretreatment, storage, and chemical treatment processes. Conversion rates and sulfur isotopic fractionations during sulfur extraction, purification, and conversion processes must be verified by researchers using standard or known samples. The unification of absence of isotopic fractionation is needed during all steps, and long-term monitoring of standard samples is recommended. This review compiles more details on different methods in sampling, laboratory operation, and measurement of sulfur isotopes, which is beneficial for researchers' better practice in laboratories. Microanalyses and molecular studies are the frontier techniques that compare the bulk sample with the elemental analysis/continuous flow-gas source stable isotope ratio mass spectrometry method, but the latter is widely used. The development of sulfur isotopic measurements will lead to the innovation in scientific issues with sulfur proxies.

On some novel solitonic structures for the Zhiber–Shabat model in modern physics

Abstract In this article, the modified Kudryashov and extended simple equation methods are employed to obtain analytical solutions for the Zhiber–Shabat problem. The outcomes of this study clearly indicate that the provided methodologies are appropriate techniques for generating some new exact solutions for nonlinear evolution equations. Furthermore, the nature of the solutions would be presented in three dimensions for various parameters applying the most advanced scientific instruments. The physical behavior of the solutions are graphically displayed, and it is established that the acquired solutions are newly constructed in the form of bright, dark, optical, singular, and bell-shaped periodic soliton wave structures. The properties of the nonlinear model have been illustrated using 3D, 2D, and contour plots by selecting an appropriate set of parameters, which is demonstrated to visualize the physical structures more productively. Finally, it is concluded that similar strategies can also be implemented to study many contemporary models. To the best of our knowledge, the current work presents a novel case study that has not been previously studied in order to generate several new solutions to the governing model appearing in diverse disciplines. The results show that the strategies that have been employed are more effective and capable than the traditional methods found in previous research.

Mass spectrometry based proteomics: Changing the impact of protein analysis in forensic science

AbstractMass spectrometry‐based proteomics, born from the invention of soft ionization, has revolutionized protein analysis. In‐depth characterization of complex and variable mixtures of proteins, such as that found at crime‐scenes, is now possible. This capability continues to expand with advancements in mass spectral instrumentation and computational power, allowing for the development of dynamic data acquisition approaches and sophisticated bioinformatics tools for data interpretation. Proteins are the major component of all biological material, as a result they have been of interest to forensic practitioners for centuries. While DNA is the gold standard for identification, proteins are able to provide contextual information about biological material not available through genomic approaches alone. Mass spectrometry‐based proteomics has expanded the breadth and ease of forensic protein analysis with several emerging applications that promise to become common place as investigative tools, particularly in forensic serology and toxicology. This includes the targeted analysis of protein biomarkers for the classification of biological tissue and fluids, the detection of protein toxins such as ricin and identification of peptide hormones such as insulin. The ability of protein analysis to leverage information from the genome has been demonstrated to achieve individual identification from biological material, an emerging technology that can be used in the absence of extractable DNA. In recent years, there has been considerable scrutiny of forensic techniques that lack objectivity and a statistical basis, an alternative molecular approach for forensic analysis that utilizes the high specificity of mass spectrometry, is a welcome addition with many potential future applications.This article is categorized under: Forensic Chemistry and Trace Evidence &gt; Trace Evidence

Imaging for ion beam therapy: current trends and future perspectives

Abstract Purpose Since the pioneering use of planar X-ray imaging in early experimental sites of proton and light ion cancer therapy, imaging has always been a cornerstone of ion beam therapy (IBT). This contribution highlights current trends and future perspectives of imaging in modern IBT. Methods Several flavours of image guidance are under investigation to enhance IBT. A first class of in-room imaging techniques aims at providing insights on updated patient anatomy prior to or ideally during treatment. Owing to the unique characteristics of IBT, these methods do not only target a correct localization of the tumour and critical structures as in photon therapy, but also aim at extracting the tissue stopping properties for accurate (re)planning. A second class of techniques, predominantly performed during beam delivery, aims at capturing different secondary emissions induced by the irradiation to identify the beam stopping position and ideally reconstruct the dose delivery for inter- or intra-fractional treatment adaptation. Finally, a third class of imaging techniques is being explored to provide novel insights on the underlying biological mechanisms to open new opportunities for more effective and better tolerated treatments. Results and conclusions 70 years after the worldwide first proton treatment, image guidance of IBT continues to be an evolving area which combines advanced instrumentation with progress in computational areas, including artificial intelligence, and beam delivery schemes. Especially on-site imaging opens new opportunities to innovate the IBT chain with daily treatment adaptation, real-time verification of in-vivo range and dose delivery along with biological guidance for treatment personalization.